Comparison of factor VIII-related von Willebrand factor proteins prepared from human cryoprecipitate and factor VIII concentrate
نویسندگان
چکیده
منابع مشابه
Comparison of factor VIII-related von Willebrand factor proteins prepared from human cryoprecipitate and factor VIII concentrate.
Both commercial factor VIII concentrate and cryoprecipitate have factor VIII coagulant activity and factor VIII antigen (VIIIR:Ag). but the concentrate lacks the von Willebrand activity (VIIIR:WF) found in the cryoprecipitate. In an effort to find a possible cause for this anomaly we compared the structures of VlllR:WF proteins responsible for the VllIR:Ag and VlllR:WF activities obtained from ...
متن کاملComparison of Factor VIlI-Related von Willebrand Factor Proteins Prepared From Human Cryoprecipitate and Factor VIII Concentrate
Both commercial factor VIII concentrate and cryoprecipitate have factor VIII coagulant activity and factor VIII antigen (VIIIR:Ag). but the concentrate lacks the von Willebrand activity (VIIIR:WF) found in the cryoprecipitate. In an effort to find a possible cause for this anomaly we compared the structures of VlllR:WF proteins responsible for the VllIR:Ag and VlllR:WF activities obtained from ...
متن کاملTopography of the human factor VIII-von Willebrand factor complex.
Factor VIII circulates in noncovalent complex with von Willebrand factor (vWf). The topography of this complex was evaluated by fluorescence energy transfer using factor VIII subunits modified with N-(1-pyrenyl)maleimide (NPM; fluorescence donor) and vWf-derived fragments modified with 7-diethylamino-3-[4'-maleimidylphenyl]-4-methyl coumarin (CPM; fluorescence acceptor). Results from a previous...
متن کاملSubcellular platelet factor VIII antigen and von Willebrand factor
Subcellular membrane and granule fractions derived from human platelets contain factor VIIII antigen and von Willebrand factor activity but not factor VII procoagulant activity. Circulating platelets constitute a significant reservoir of plasma factor VIII antigen, containing approximately 15% of the amount of factor VIII antigen present in platelet-poor plasma. The antibiotic ristocetin, which...
متن کاملIsolation and characterization of human factor VIII: molecular forms in commercial factor VIII concentrate, cryoprecipitate, and plasma.
Human factor VIII has been isolated from a high purity factor VIII concentrate by immunoaffinity chromatography and HPLC on Mono Q gel. Two fractions of factor VIII were obtained with a specific activity of approximately equal to 7000 units/mg. The major fraction contained eight peptide chains of 200, 180, 160, 150, 135, 130, 115, and 105 kDa plus one doublet chain of 80 kDa. The minor fraction...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Blood
سال: 1979
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v53.6.1095.1095